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DAD Detector HPLC Liquid Chromatography Instrument In Quantitative Analysis

Categories Liquid Chromatography Instrument
Brand Name: Wayeal
Model Number: LC3200
Certification: ISO, CE
Place of Origin: Anhui, China
MOQ: 1
Price: Negotiation
Payment Terms: L/C, T/T
Supply Ability: 30 Pieces Per Month
Delivery Time: 30 days
Packaging Details: Wooden Case
Product Name: HPLC Liquid Chromatography Instrument
Detector: DAD/UVD/ELSD/FLD/RID
Linearity: ≥1.0×104
Wavelength Accuracy: 250μL( Standard, with 500μL,1000μL, 2500μL optionals)
Wavelength Range: 190-800nm
LOD: 5.0×10-9g/mL (naphthalene/methanol)
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DAD Detector HPLC Liquid Chromatography Instrument In Quantitative Analysis

DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Qualitative and Quantitative Analysis

Description of Liquid Chromatography Instrument


High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure. HPLCinstruments consist of a reservoir of mobilephase, a pump, an injector, a separation column, and adetector. Compounds are separated by injecting a sample mixtureonto the column. The different component in the mixture pass through thecolumn atdifferentiates due to differencesintheirpartition behavior between the mobile phase and thestationary phase. The mobile phase must be degassed to eliminate theformation of air bubbles.


Parameters of Liquid Chromatography Instrument


DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Quantitative Analysis
Physical Specification
Power Requirements220V,50HZ
Ambient Temperature4-40℃
Ambient Humidity20-85% relative humidity(non-condensing)
System
Qualitative repeatability(Retention time)RSD≤0.3%
Quantitative repeatability(Area)RSD≤0.6%
Pump
Flow Rate Range0.0001-10.000mL/min( increments :0.0001 mL/min)
Max Operating Pressure42MPa
Pressure Pulsation<0.1MPa( Test condition: 10MPa , at 1.0ml/min methanol)
Flow Accuracy±0.2%( Test condition: 10MPa , at 1.0ml/min methanol)
Flow PrecisionRSD≤0.06%( Test condition: 10MPa , at 1.0ml/min methanol)
Gradient Accuracy±1%
Column Oven
Temperature Setting Range5 °C ~ 85 °C
Temperature Stability≤0.2°C/h
Temperature Controlling Precision±0.1 °C
Autosampler
Injection Volume Precision

<0.25%RSD( full loop) ;

<0.5%RSD( partial loop) ;

<1%RSD( no waste)

Linearity>0.999
Cross Contamination(Carry Over)<0.01%
Sample Capacity120 Samples( 1.5ml vials)
Syringe250 μL( Standard, with 500μL,1000μL, 2500μL optionals)
Sample Loop20μL( Standard, with 50μL, 100μL, 200μL optional)

DAD Detector

Noise1.5×10-5Au
Drift≤1×10-3Au/h
LOD5.0×10-9g/mL (naphthalene/methanol)
Wavelength Range190-800nm
Wavelength Reproducibility≤±0.1 nm
Linearity≥1.0×104







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