20 50preps RNA DNA Extraction Kit , Column Purified RNA / DNA Purification Micro Kit

Purification is based on spin column chromatography. The process involves first lysing the cells or tissue of interest with the provided Buffer SKP. For hard-to-extract fibrous tissues (such as heart and muscle), an additional Proteinase K digestion step is provided for maximum recovery of the RNA and DNA. The DNA in the lysate is then captured and purified on a gDNA Purification Micro Column. Ethanol is then added to the flowthrough of the DNA purification step, and the solution is loaded onto an RNA Purification Micro Column. Bioteke’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus only the RNA including microRNAs will bind to the column while the proteins are removed in the flowthrough. Next, the bound RNA is washed with the provided Wash Solution A to remove impurities, and the purified RNA is eluted with the Elution Solution A. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Both RNA and DNA fractions can be eluted in as little as 20 L, resulting in a higher concentration of nucleic acids. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The genomic DNA is of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting, methylation studies and SNP analysis.