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Recombinant Proteinase K

Country/Region china
Company Shanghai Yaxin Biotechnology Co.,Ltd
Categories Proteinase K
Telephone +86-21-54336592
ICP License Issued by the Chinese Ministry
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    Recombinant Proteinase K

    ProductsRecombinant Enzymes

    Recombinant Proteinase K
    Instructions Download

    Recombinant Proteinase K
    Catalog Number:RPK09
    Cas:39450-01-6
    EC:3.4.21.14
    Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
    DESCRIPTION
    A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
    ADVANTAGES
    FormatLyophilized
    M.W29.8kD
    Purity99%
    Activity30 units/mg
    DNAses and RNAsesnot detected

    UNIT DEFINITION
    One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
    RECOMMEND USAGE
    Prepare recombinant Proteinase K with 50 mM Tris-HCl,2 mM Calcium acetate.The optimum pH is 8.0.
    STORAGE INSTRUCTIONS
    Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
    HANDLING
    Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
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