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Proteinase K, Solution(20mg/ml)

Proteinase K, Solution(20mg/ml)
Country/Region china
Company Guangzhou Geneshun Biotech Ltd
Categories Beakers
Telephone 020-87056567
ICP License Issued by the Chinese Ministry
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    Proteinase K, Solution(20mg/ml)

    Proteinase K Solution (20mg/ml)

    Cat# ES10711ml
    Grade:
    PCR grade, for Molecular Biology
    Storage Temperature 20°C
    Description
    Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.The recommended working concentration for Proteinase K is 0.05-1 mg/ml.
    Applications
    <1> for isolation of genomic DNA from different kind of samples.
    <2> for isolation of total RNA from different kind of samples.
    Inhibition and Inactivation

    Inhibitors: Proteinase K is not inactivated by metal chelators, by thiol-reactive reagents or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.
    Inactivated by heating at 95°C for 10 minutes.

    Note:

    Optimum activity at 50-55°C.
    Rapid denaturation of enzyme occurs at temperatures above 65°C.
    The recommended working concentration for Proteinase K is 0.05-1 mg/ml. The activity of the enzyme is stimulated by 0.2-1% SDS or by 1-4 M urea.
    Ca2+protects Proteinase K against autolysis, increases the thermal stability and has a regulatory function for the substrate binding site of Proteinase K .Stable over a wide pH range: 4.0-12.5, optimum pH 7.5-8.0 .

    Product Tags:

    amino acids

      

    10 minutes

      

    binding site

      
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